Photoeradication of microorganisms with pulsed purple or blue light

A method for photoeradication of microorganisms from a target, the method comprising:

• determining a plurality of pulse parameters for a sequence of light pulses having a wavelength that ranges from 380 nm to 500 nm, wherein the pulse parameters comprise (a) a peak irradiance and a pulse duration for each of the light pulses that are selected to enable excitation of a photoactive molecule present in the microorganisms and (b) an off time between each two adjacent light pulses that is selected to enable return of the photoactive molecule to a ground state to thereby create an oxidation reaction that produces free radicals which destroy a cellular structure of the microorganisms;
• determining an irradiation schedule for application of the light pulses having the pulse parameters during each of a plurality of irradiation sessions separated by one or more recovery periods, wherein the irradiation sessions are timed to target the microorganisms at a specified time during a replication cycle of the microorganisms;
• and
• irradiating the microorganisms of the target with the light pulses having the pulse parameters in accordance with the irradiation schedule.