Development of YY male fish broodstocks in a single generation

A method of creating a YY animal broodstock, wherein the broodstock includes only sperm-producing YY males and egg-producing YY males, the method comprising:

• (a) using androgenesis to create a first generation of juvenile XX females and juvenile YY males;
• (b) exposing a predetermined portion of the first generation created in step (a) to a feminizing hormone while the first generation created in step (a) are still juveniles, such that following step (b), a post-exposure first generation includes juvenile XX females and juvenile YY males in which at least some of the juvenile XX females and at least some of the juvenile YY males therein have been exposed to the feminizing hormone;
• and
• (c) following step (b), using genetic sex marker testing to screen the post-exposure first generation for, and separate out, future sperm-producing YY males and future egg-producing YY males, thereby creating a YY animal broodstock.

A method of creating a YY animal broodstock, wherein the broodstock includes only sperm-producing YY males and egg-producing YY males, the method comprising:

• (a) using androgenesis to create a first generation of juvenile XX females and juvenile YY males;
• (b) exposing a predetermined portion of the first generation created in step (a) to a feminizing hormone while the first generation created in step (a) are still juveniles, such that following step (b), a post-exposure first generation includes juvenile XX females and juvenile YY males in which at least some of the juvenile XX females and at least some of the juvenile YY males therein have been exposed to the feminizing hormone;
• and
• (c) following step (b), and while the first generation are still juveniles, using genetic sex marker testing to screen the post-exposure first generation for, and separate out, future sperm-producing YY males and future egg-producing YY males, thereby creating a YY animal broodstock.