Bioresorbable exopolysaccharides
AssigneeDePuy Synthes Products, Inc.
InventorDouglas D. Buechter
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Abstract
The present disclosure is directed to microorganisms that are genetically modified to express at least one exogenous enzyme involved in D-glucosamine uptake and metabolism. Methods for the production of an exopolysaccharide are also disclosed. The exopolysaccharide comprises N-acetylglucosamine and D-glucose and may be used as a bioresorbable implant for soft tissue repair, replacement, or augmentation.
Claims11
A bacterium for the production of an exopolysaccharide comprising N-acetylglucosamine and D-glucose, wherein:
- the bacterium belongs to a genus comprising Komagataeibacter, Agrobacterium, Aerobacter, Achromobacter, Azotobacter, Rhizobium, Sarcina, or Salmonella and the bacterium expresses an exogenous
- Bacillus subtilis phosphoenolpyruvate:sugar phosphotransferase system glucosamine specific transporter subunit EIICBA (GamP);
- and wherein,
- the bacterium produces an exopolysaccharide that comprises N-acetylglucosamine and D-glucose.
A bacterium for the production of an exopolysaccharide comprising N-acetylglucosamine and D-glucose, wherein:
- the bacterium belongs to a genus comprising Komagataeibacter, Agrobacterium, Aerobacter, Achromobacter, Azotobacter, Rhizobium, Sarcina, or Salmonella and the bacterium expresses an exogenous
- a. Bacillus subtilis phosphoenolpyruvate:sugar phosphotransferase system glucosamine specific transporter subunit EIICBA (GamP);
- and
- b. Escherichia coli phosphoglucosamine mutase, UDP-N-acetylglucosamine pyrophosphorylase, and glucosamine-1-phosphate N-acetyltransferase;
- and wherein
- the bacterium produces an exopolysaccharide that comprises N-acetylglucosamine and D-glucose.
Dependencies are inferred from the claim text.