Recombinant cells and methods for biosynthesis of ent-atiserenoic acid
AssigneeRegents of the University of Minnesota
InventorsMichael Joseph Smanski, Szu-Yi Hsu
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Abstract
This disclosure describes recombinant cells and methods for microbial biosynthesis of ent-atiserenoic acid. Thus, in one aspect, this disclosure describes a recombinant cell genetically modified to exhibit increased biosynthesis of ent-atiserenoic acid compared to a comparable control cell. In some cases, the recombinant cell can include a host cell modified to include at least one heterologous polynucleotide encoding at least one enzyme in a biosynthetic pathway that produces ent-atiserenoic acid. In some cases, the recombinant cell can include a host cell and at least one heterologous enzyme in a biosynthetic pathway that produces ent-atiserenoic acid.
Claims5
A recombinant cell comprising a host cell genetically modified to comprise heterologous polynucleotides that encode:
- a polyprenyl diphosphate synthase;
- a type II terpene cyclase;
- a type I terpene cyclase;
- a p450 monooxygenase;
- and
- a ferredoxin;
- wherein the recombinant cell biosynthesizes ent-atiserenoic acid compared to a host cell that lacks the genetic modification of the recombinant cell.
Dependencies are inferred from the claim text.