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US 10,633,650 B2Granted
Expression and secretion system
AssigneeGenentech, Inc.
InventorsDevin Tesar, Xiaocheng Chen, Mark Dennis, Isidro Hotzel
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Abstract
The invention provides an expression and secretion system, and methods of using the same, for the expression and secretion of one fusion protein in prokaryotic cells and a second fusion protein in eukaryotic cells. Also provided herein are nucleic acid molecules, vectors and host cells comprising such vectors and nucleic acid molecules.
Claims19
A nucleic acid molecule comprising:
- (a) a variable light chain (VL) domain-encoding sequence that encodes a VL domain, wherein the VL domain-encoding sequence is 3′ to a first signal sequence that encodes a first signal peptide;
- (b) a variable heavy chain (VH) domain-encoding sequence that encodes a VH domain, wherein the VH domain-encoding sequence is 3′ to a second signal sequence that encodes a second signal peptide, wherein each of the first signal peptide and the second signal peptide is functional in both a prokaryotic cell and a eukaryotic cell;
- (c) an Fc region-encoding sequence that encodes an Fc region;
- (d) a prokaryotic promoter and a eukaryotic promoter, wherein the prokaryotic promoter and the eukaryotic promoter are each operably linked to the VH domain-encoding sequence and/or the VL domain-encoding sequence;
- (e) a splice donor 3′ to the VH domain-encoding sequence;
- and
- (f) a splice acceptor 5′ to the Fc region-encoding sequence.
4 more elements
Dependencies are inferred from the claim text.