Coated substrate for biological reaction systems
AssigneeLife Technologies Corporation
InventorsEliodor Ghenciu, Theodore E. Straub, Evan W. Foster, Michael C. Pallas
Life of this patent
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Abstract
An apparatus for biological reactions is provided. The apparatus includes a substrate and a plurality of reaction sites within the substrate. A surface of the substrate is configured to have a first hydrophilicity and each surface of the plurality of reaction sites is configured to have a second hydrophilicity to load a substantial number of reaction sites with a sample volume. The sample volume of each loaded reaction site is substantially confined to its respective reaction site. The sample volume is configured to undergo a biological reaction within the reaction site.
Claims20
An apparatus for biological reactions, the apparatus comprising:
- a substrate comprising a substrate surface;
- and
- a plurality of reaction sites disposed along the substrate surface, each reaction site spatially separated from the remaining reaction sites, each reaction site comprising a reaction site surface;
- a coating material, wherein:
- the coating material coats the substrate surface, to provide a coated substrate surface;
- the coating material coats each of the reaction site surfaces to provide a plurality of coated reaction sites;
- the coated substrate surface and the coated reaction site surfaces are hydrophilic;
- the coated substrate surface has a water contact angle of 60-90 degrees;
- and
- the coated substrate surface has an advancing contact angle of 70-85 degrees;
- wherein each of the coated reaction site surfaces is configured to load the reaction sites with a sample;
- and
- wherein each sample is configured to undergo a biological reaction within the reaction site.
The apparatus of claim wherein the hysteresis of the substrate surface is zero degrees or is between 0-15 degrees.
An apparatus for biological reactions, the apparatus comprising:
- a loader configured to receive a sample;
- a substrate comprising a substrate surface;
- and
- a plurality of reaction sites disposed along the substrate surface, each reaction site spatially separated from the remaining reaction sites, each reaction site comprising a reaction site surface;
- a coating material, wherein:
- the coating material coats the substrate surface to provide a coated substrate surface;
- the coating material coats each of the reaction site surfaces to provide a plurality of coated reaction sites;
- the coated substrate surface and the coated reaction site surfaces are hydrophilic;
- the coated substrate surface has a water contact angle of 60-90 degrees:,
- the coated substrate surface has an advancing contact angle of 70-85 degrees;
- and
- the substrate surface has a hysteresis of less than 30 degrees;
- wherein the loader is configured to contact the substrate surface to load the sample into at least some of the reaction sites of the substrate such that all of the sample is disposed within the reaction sites and none of the sample is contained on the substrate surface;
- and
- wherein each sample is configured to undergo a biological reaction within the reaction site.
An apparatus for biological reactions, the apparatus comprising:
- a substrate comprising a substrate surface;
- and
- a plurality of reaction sites disposed along the substrate surface, each reaction site spatially separated from the remaining reaction sites, each reaction site comprising a reaction site surface;
- a coating material, wherein:
- the coating material coats the substrate surface to provide a coated substrate surface:
- the coating material coats each of the reaction site surfaces to provide a plurality of coated reaction sites;
- the coated substrate surface and the coated reaction site suffixes are hydrophilic;
- the coated substrate surface has a water contact angle of 60-90 degrees;
- the coated substrate surface is characterized by an advancing contact angle of 70-85 degrees;
- and
- wherein the coated substrate surface is characterized by a hysteresis of less than 30 degrees;
- wherein each of the coated reaction site surfaces is configured to load the reaction sites with a sample;
- and
- wherein each sample is configured to undergo a biological reaction within the reaction site.
Dependencies are inferred from the claim text.