# US 10,626,407 B2 · Materials and method for modifying a biochemical component in a plant

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- Number: US 10,626,407 B2
- Document ID: US10626407B2
- Status: Granted
- Kind: B2
- Application number: 15/820,372
- Filed: 2017-11-21
- Published: 2018-04-19
- Granted: 2020-04-21
- Earliest claimed: 2011-02-24
- Assignees: Iowa State University Research Foundation, Inc.
- Applicants: Iowa State University Research Foundation, Inc.
- Inventors: Ling Li; Eve Syrkin Wurtele
- Web page: https://patentgrep.com/patent/US10626407B2

## CPC

- C12N 15/8245: {involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis}
- C07K 14/415: from plants
- C12N 15/8234: {Seed-specific, e.g. embryo, endosperm}
- C12N 15/8247: {involving modified lipid metabolism, e.g. seed oil composition}
- C12N 15/8251: {Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis}
- C12N 2710/00043: viral genome or elements thereof as genetic vector
- C12N 2830/008: cell type or tissue specific enhancer/promoter combination
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

A method of modifying the amount of at least one biochemical component in a plant comprising expressing Qua-Quine Starch (QQS) in the plant, the wild-type of which does not express QQS; a transgenic plant, or part thereof, which comprises and expresses QQS as a transgene and in which the amount of at least one biochemical component is modified; a tissue culture of regenerable cells of the transgenic plant; a vector comprising a nucleotide sequence, which encodes the coding sequence of QQS, operably linked to a non-native promoter, which promotes expression of the nucleotide sequence in a plant, which is other than Arabidopsis; and a method of producing a food or industrial product from a plant.

## Claims

### Claim 1

A method of producing a food product with increased protein content comprising:
(a) obtaining a transgenic plant or a transgenic part thereof, wherein the transgenic plant or the transgenic part thereof is transformed with a polynucleotide, which encodes an Arabidopsis Qua-Quine Starch (QQS) polypeptide, operably linked to a non-native promoter that expresses the QQS polypeptide in the transgenic plant or the transgenic part thereof;
(b) selecting the transgenic plant or the transgenic part thereof with increased total protein content compared to the total protein content in a corresponding wild-type (WT) plant or a corresponding WT part thereof that does not comprise and express the polynucleotide, which encodes the Arabidopsis QQS polypeptide, operably linked to the non-native promoter; and
(c) preparing the food product from the transgenic plant or the transgenic part thereof with increased total protein content,
whereupon the food product with increased protein content is produced.

### Claim 2 (depends on 1)

The method of claim 1, wherein the transgenic plant is a crop plant.

### Claim 3 (depends on 2)

The method of claim 2, wherein the crop plant is a cereal crop.

### Claim 4 (depends on 3)

The method of claim 3, wherein the cereal crop is corn.

### Claim 5 (depends on 3)

The method of claim 3, wherein the cereal crop is rice.

### Claim 6 (depends on 2)

The method of claim 2, wherein the crop plant is soybean.