Production of viruses in cell culture
AssigneesCommonwealth Scientific and Industrial Research Organisation, University of Georgia Research Foundation, Inc.
InventorsAndrew Bean, John William Lowenthal, Luis Fernando Malaver-Ortega, Ralph A. Tripp
Life of this patent
Estimating the term…
Abstract
The present invention relates to methods of replicating viruses in vitro. In particular, the invention relates to a genetically modified population of cells, and/or a population of cells treated with an exogenous compound, wherein the cells are capable of producing more virus than cells lacking the genetic modification and/or lacking treatment with the exogenous compound. The invention also relates to methods of producing populations of such cells, as well as the use of the viruses obtained to prepare vaccine compositions.
Claims20
A method of replicating a virus, the method comprising
- 1) inoculating a population of cells with a virus in vitro, the cells having a genetic modification which reduces the expression of the Interleukin 1 receptor type 1 (IL-1RA) gene in the cells by at least 50% when compared to isogenic cells lacking the genetic modification;
- and
- 2) culturing the cells for a predetermined period of time to replicate the virus, wherein the cells are capable of producing more virus than a population of the isogenic cells wherein the virus is an Orthomyxoviridae virus.
Dependencies are inferred from the claim text.