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US 10,626,149 B2Granted
Viral chimeric particle of potato virus X and use thereof for in vitro diagnosis of Sjögren syndrome
AssigneesENEA—Agenzia Nazionale per le Nuove Tecnologie, l'Energia e lo Sviluppo Economico Sostenibile, Universita' Degli Studi Di Verona
InventorsChiara Lico, Selene Baschieri, Eugenio Benvenuto, Linda Avesani, Elisa Tinazzi, Elena Bartoloni Bocci, Mario Pezzotti, Claudio Lunardi
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Abstract
The present invention relates to a chimeric virus particle of potato virus X, said particle having, as a capsid protein, a fusion protein containing a capsid protein and an antigenic determinant of lipocalin, and the use thereof in the in vitro diagnosis of Sjögren's Syndrome using the ELISA method.
Claims6
A method for in vitro diagnosis of Sjögren's Syndrome, the method comprising
- providing a chimeric virus particle;
- and
- ascertaining presence of autoantibodies against lipocalin;
- wherein the chimeric virus particle comprises, as a capsid protein, a fusion protein comprising an amino-terminal portion consisting of a peptide sequence comprising an antigenic determinant of lipocalin and a carboxy-terminal portion consisting of a peptide sequence comprising a PVX virus capsid protein,
- wherein the PVX virus capsid protein is encoded by a wild-type capsid protein gene that is intact or deleted in the 5′ portion of the gene,
- wherein the amino-terminal portion is fused to the carboxy-terminal portion such that the antigenic determinant is in frame with the capsid protein, and
- wherein the antigenic determinant is FEKAAGARGLST (SEQ ID NO:2) or MSFEKAAGARGLST (SEQ ID NO:5).
4 more elements
Dependencies are inferred from the claim text.