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US 10,619,172 B2Granted
Increased ethanol production by thermophilic microorganisms with deletion of individual hfs hydrogenase subunits
AssigneeTrustees of Dartmouth College
InventorsAysenur Eminoglu, Daniel G. Olson, Lee R. Lynd
Life of this patent
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Abstract
Disclosed are methods for engineering bacteria, for example, Thermoanaerobacterium saccharolyticum, that convert biomass to ethanol at high yield by deleting a single gene. Deletion of subunit A or subunit B of the hfs hydrogenase, but not deletion of subunit C or subunit D, results in an increase in ethanol yield.
Claims12
A genetically engineered microorganism comprising a functional hfsC protein subunit and a functional hfsD protein subunit of hfs hydrogenase, wherein expression of hfsA and hfsB protein subunits of the hfs hydrogenase is disrupted,
- wherein said microorganism is selected from the group consisting of Thermoanaerobacterium sp., Thermoanaerobacter mathranii and Clostridium thermocellum.
Dependencies are inferred from the claim text.