Method for crystallizing protein

A method for crystallizing protein using a protein crystal device,

• the protein crystal device comprising:
• a protein-adsorbing-bubble-jetting member, a counter electrode, and an electricity output means,
• the protein-adsorbing-bubble-jetting member comprising:
• a core formed of a conductive material;
• a shell part formed of an insulating material, the shell part including an extended section extending from a tip of the core, and at least a portion closely adhering to the core to cover the core;
• a gap having a bubble-jetting port, the gap being formed between the extended section and the tip of the core;
• and
• an outside shell part on the outside of the shell part;
• wherein
• the outside shell part has an axis coaxial with the center axis of the shell part,
• the outside shell part is formed at a position away from the shell part so that a space is left therebetween, and
• the outside shell part has a protein-adsorbing-bubble-jetting port,
• the method for crystallizing protein comprising:
• a step for introducing a protein solution into the space between the outside shell part and the shell part of the protein-adsorbing-bubble-jetting member of the protein crystal device and/or the space formed by the outside shell part and a tip of the extended section of the shell part;
• a step for immersing at least the protein-adsorbing-bubble-jetting port of the protein-adsorbing-bubble-jetting member in a protein solution, buffer solution, or precipitant-containing solution, and forming a state in which the core of the protein-adsorbing-bubble-jetting member and the counter electrode can be energized;
• and
• a step for outputting electricity to a pair of electrodes constituted by the counter electrode and the core of the protein-adsorbing-bubble-jetting member, and jetting bubbles, in which protein and/or a protein solution has been adsorbed onto the boundary, from the protein-adsorbing-bubble-jetting port into the protein solution, buffer solution, or precipitant-containing solution.