Vibrio assay methods and kits

A Vibrio detection assay vessel for detecting the presence of a target Vibrio bacteria consisting essentially of:

• at least one solid-phase gel medium and a matrix material allowing facultative anaerobic growth of the target Vibrio bacteria, a liquid-phase medium, and a visible signal indicator within the at least one solid-phase gel medium disposed in a single culture vessel;
• wherein the at least one solid-phase gel medium reduces false-positive errors in detecting Vibrio bacteria and suppresses growth of non-target bacteria and wherein the liquid-phase medium increases the sensitivity of the Vibrio bacteria detection assay vessels evidenced by altering the characteristics of said signal indicator within the at least one solid phase medium reduces false-negative errors in detecting Vibrio bacteria and enhance growth of the target Vibrio bacteria;
• further wherein the target Vibrio bacteria detected within 24 hours or less of inoculating the single culture vessel without the use of additional machinery or equipment is selected from the group consisting of Vibrio parahaemolyticus and Vibrio vulnificus.