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US 10,604,773 B2Granted
Cells with improved pentose conversion
AssigneeDsm IP Assets B.V.
InventorsMaria Bernedina Elizabeth Jonkers, Paul Klaassen, Aloysius Wilhelmus Rudolphus Hubertus Teunissen, Rene Marcel De Jong
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Abstract
The invention relates to a cell capable of converting one or more pentose sugar and one or more hexose sugar into fermentation product constitutively expressing one or more heterologous or homologous polypeptide having the amino acid sequence set out in SEQ ID NO: 20, or a variant polypeptide thereof having at least 45% identity to SEQ ID NO 20. In an embodiment the heterologous polypeptide has glyoxalase activity.
Claims20
A Saccharomyces cell comprising
- i) a heterologous nucleotide sequence encoding a xylose isomerase;
- ii) at least one heterologous or homologous nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:
- 20, or encoding a variant polypeptide thereof having at least 45% sequence identity to SEQ ID NO:
- 20, wherein the nucleotide sequence encoding SEQ ID NO:
- 20 or the variant polypeptide is constitutively expressed;
- and
- iii) an inactivated endogenous aldose reductase gene,
- wherein the cell is capable of converting one or more pentose sugars and one or more hexose sugars into a fermentation product.
5 more elements
A Saccharomyces cell comprising:
- (a) the nucleotide sequence as set forth in SEQ ID NO:
- 27;
- or
- (b) a codon-pair optimized nucleotide sequence encoding SEQ ID NO:
- 20 for expression in the Saccharomyces,
- wherein the nucleotide sequence in (a) or (b) is constitutively expressed in the cell, wherein the cell further comprises an inactivated endogenous aldose reductase gene, and wherein the cell is capable of converting one or more pentose sugars and or one or more hexose sugars into a fermentation product.
3 more elements
A Saccharomyces cerevisiae cell comprising
- i) a heterologous nucleotide sequence encoding a xylose isomerase;
- ii) at least one heterologous or homologous nucleotide sequence encoding SEQ ID NO:
- 20, wherein the nucleotide sequence encoding SEQ ID NO:
- 20 is constitutively expressed;
- iii) an inactivated GRE3 gene;
- iv) overexpression of the genes TAL1, TKL1, RPE1 and RKI1;
- v) a heterologous nucleotide sequence encoding one or more of the araA, araB and araD gene;
- and
- vi) overexpression of a homologous or heterologous nucleotide sequence encoding a xylulose kinase
- wherein the cell is capable of converting xylose or arabionse into ethanol.
7 more elements
Dependencies are inferred from the claim text.