Cell classification based on mechanical signature of nucleus
AssigneesUniversity of Maryland, Canon U.S.A., Inc.
InventorsGiuliano Scarcelli, Jitao Zhang, Hanyoup Kim
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Abstract
The present invention relates to a method and system for identifying mechanical properties of a cell nucleus through a label-free cell analysis based on Brillouin light scattering techniques. The present application additionally provides a method and system for identifying cancerous cells based on mechanical properties of the cell nucleus.
Claims23
A method for determining a mechanical property of a nucleus of at least one cell in a sample comprising:
- acquiring the at least one cell's mechanical signatures using label-free cytometry enabled by Brillouin spectroscopy;
- and
- classifying cells based on the cells mechanical signatures of the at least one nuclei.
A system for obtaining one or more Brillouin metrics associated with a Brillouin scattering spectrum at multiple points, wherein a least one point is within a nucleus of at least one cell in a sample, the system comprising:
- an illumination source illuminating the sample by a light beam along a first direction;
- one or more lenses collecting a Brillouin scattered light emitted from the sample in response to the illuminating light beam;
- an optical arrangement to receive the Brillouin scattered light from the one or more lenses, the optical arrangement to induce a spectral dispersion;
- a detection unit to detect a spatio-spectral pattern of the Brillouin scattered light, wherein the one or more lenses, the optical arrangement, and the detection unit are positioned along a second direction, wherein multiple points of the sample along the illuminating light beam are measured simultaneously;
- a processor executing instructions for:
- calibrating the spectral pattern at each spatial point at the detection unit;
- and
- calculating the one or more Brillouin metrics at each measured sample point based on the detected spatio-spectral pattern.
A method of identifying a cancerous cell in a sample, comprising:
- illuminating the sample by a light beam along a first direction;
- collecting a Brillouin scattered light emitted from the sample in response to the illuminating light beam;
- sending the Brillouin scattered light to an optical arrangement to induce a spectral dispersion and to a detection unit to generate a spatio-spectral pattern of the Brillouin scattered light, wherein the optical arrangement and the detection unit are positioned along a second direction;
- detecting the spatio-spectral pattern of the Brillouin scattering light onto the detection unit, wherein multiple points of the sample along the illuminating light beam are measured simultaneously;
- wherein at least one of the multiple points of the sample is a cell nucleus;
- calibrating the spectral pattern at each spatial point at the detection unit;
- and calculating the one or more Brillouin metrics at each measured sample point based on the detected spatio-spectral pattern;
- identifying cancerous cells present in the sample by comparing the calculated Brillouin metrics to those of normal cells.
Dependencies are inferred from the claim text.