Automated priming and library loading device

A nucleic acid sequencing apparatus comprising:

• (a) a nucleic acid library preparation compartment comprising two or more chambers configured to prepare a plurality of nucleic acids from a sample for a sequencing reaction, wherein said compartment is operatively connected to a nucleic acid sequencing chamber;
• (b) a nucleic acid sequencing chamber, wherein said nucleic acid sequencing chamber comprises:
• (i) one or more flow cells comprising a plurality of pores or sequencing cartridges configured for the passage of a nucleic acid strand, wherein each said one or more flow cells comprises a sample input port comprising a flow path in fluid communication with a sensing chamber outlet, wherein said sample input port is configured to be robotically opened, wherein said sample input port comprises a plug that projects a sufficient distance above the surface of the flow cell for removal by a robotic tool;
• and
• (c) an automated platform, wherein said automated platform is programmed to robotically move a sample from said nucleic acid library preparation compartment into said sample input port in said nucleic acid sequencing chamber.