Rotating culture vessel and automatic cell culture apparatus using same

An automatic cell culture apparatus using a rotating culture vessel used for three-dimensional culture of a cell in a nearly gravity-free microgravity environment, the rotating culture vessel including a flat cylindrical culture container having a cylinder axis, being removably attached to an end of a horizontal rotating shaft of a rotating culture device such that the cylinder axis is coaxially arranged to the horizontal rotating shaft as a rotating center, one or more inlets/outlets for supplying a cell and a liquid culture medium at an initial stage and taking out a cultured cell at an appropriate position on the flat cylindrical culture container, and at least one pair of a supply port and a discharge port for exchanging the liquid culture medium on an outer circumferential cylindrical face of the culture container so that the discharge port faces vertically upward when the supply port faces vertically downward, the pair of the supply port and the discharge port being positioned vertically opposite to each other in a state where the discharge port is facing vertically upward when the supply port is facing vertically downward, the discharge port having a first center line vertically extending with passing through a center of the discharge port, the first center line passing through a center axis of the rotating shaft, which serves as the rotating center, and the supply port having a second center line vertically extending with passing through a center of the supply port, the second center line extending without passing through the center axis of the rotating shaft, which serves s the rotating center, wherein a distance between the second center line of the supply port and the rotating center is 0.5r to 0.9r where the culture container has a culture space having a radius of r, the automatic cell culture apparatus comprising:

• a closed housing having an air conditioning function;
• an incubator box of a rotating culture device in a middle chamber of the closed housing;
• a cool supply box storing a supply syringe for supplying a liquid culture medium in a lower chamber of the closed housing;
• and
• a cool discharge box storing a discharge syringe for collecting a liquid culture medium in an upper chamber of the closed housing;
• each of the incubator box, the cool supply box, and the cool discharge box including a front face having an automatically operated door,
• the rotating culture device having a shaft direction shifting means shifting a horizontal rotating shaft back and forth in a shaft direction, the horizontal rotating shaft being provided in the incubator box,
• the rotating shaft having an end removably installed with the rotating culture vessel,
• a front space of the cool supply box including an XYZ-axis shifting mechanism for supply,
• the XYZ-axis shifting mechanism for supply including a movable part for supply driven by the mechanism, the movable part for supply having a fixing chuck for holding the supply syringe upward and a push up means for pushing a piston up,
• a front space of the cool discharge box including an XYZ-axis shifting mechanism for discharge,
• the XYZ-axis shifting mechanism for discharge including a movable part for discharge driven by the mechanism, the movable part for discharge having a fixing chuck for holding the discharge syringe downward and a pull up means for pulling a piston up, and
• each door being opened, the shaft direction shifting means being driven to bring the rotating culture vessel from the incubator box to a front space, the rotating culture vessel being stopped with the supply port facing downward and the discharge port facing upward, the XYZ-axis shifting mechanism for supply and the fixing chuck being driven to take out the supply syringe from the cool supply box and to locate the supply syringe below the rotating culture vessel, the XYZ-axis shifting mechanism for discharge and the fixing chuck being driven to take out the discharge syringe from the cool discharge box and to locate the discharge syringe above the rotating culture vessel, the XYZ-axis shifting mechanism for supply being driven to airtightly connect the supply syringe to the supply port,
• simultaneously, the XYZ-axis shifting mechanism for discharge being driven to airtightly connect the discharge syringe to the discharge port, and then the push up means and the pull up means being synchronously driven to exchange a liquid culture medium in the rotating culture vessel.