Sperm inspection method and device

A non-transitory computer-readable medium storing a computer program, wherein the computer program causes a sperm inspection device which comprises a camera and a processor to execute following processes:

• capturing an image of a sperm population suspended in an inspection liquid,
• measuring a quality indicator value of individual sperm of a sperm population suspended in an inspection liquid based on the image, wherein the quality indicator value is curved line velocity (VCL);
• stratifying the sperm population based on the quality indicator, and
• evaluating sperm quality based on the quality indicator value of a high quality group, wherein the high quality group is within the top 10% of the stratified sperm population, wherein sperm quality is determined by comparing a quality determination value, which is the mean, median, or mode of a quality indicator value of the high quality group, or the proportion of sperm having a reference value that exceeds or is within a certain range of a reference value of a prescribed quality indicator value of the high quality group, with a reference value of the quality determination value for which a correlation with low fertility samples having conception rates of less than 40% has been indicated in advance.

A method for controlling a sperm inspection device, comprising a camera and a processor, comprising:

• operating the high speed camera to capture an image of a sperm population suspended in an inspection liquid,
• operating the processor to measure a quality indicator value of individual sperm of a sperm population suspended in an inspection liquid, based on the image, wherein the quality indicator value is curved line velocity (VCL);
• stratifying the sperm population based on the quality indicator value, and
• evaluating sperm quality based on the quality indicator value of a high quality group, wherein the high quality group is within the top 10% of the stratified sperm population, wherein sperm quality is determined by comparing a quality determination value, which is the mean, median, or mode of a quality indicator value of the high quality group, or the proportion of sperm having a reference value that exceeds or is within a certain range of a reference value of a prescribed quality indicator value of the high quality group, with a reference value of the quality determination value for which a correlation with low fertility samples having conception rates of less than 40% has been indicated in advance.