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US 10,591,497 B2Granted
Immunoassay for synthetic cannabinoids of the adamantyl indazole/indole-3-carboxamide family
AssigneeRandox Laboratories Limited
InventorsIvan McConnell, Elouard Benchikh, Philip Lowry, Peter Fitzgerald
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Abstract
An immunoassay method for detecting and determining adamantane substituted indazole and indole synthetic cannabinoids is described. Also described are components for use in implementing the method, namely, antibodies, detection agents, solid state devices and kits as well as immunogens used to raise the antibodies.
Claims8
A polyclonal antibody derived from an immunogen having the structure:
- wherein KLH is keyhole limpet hemocyanin;
- wherein the polyclonal antibody binds to one or more epitopes of one or more molecules of Structure I′,
- wherein X is H or F;
- Y is N or CH;
- and Z is H or OH;
- and wherein the polyclonal antibody has an IC50 of 0.18 ng/ml for 3-OH-AKB48, 2.08 ng/ml for AKB-48, 2.10 ng/ml for N-(5-fluoropentyl)-AKB48 and 4.40 ng/ml for 3-OH-SDB001, and <1.00% cross-reactivity with AKB48 N-Pentanoic acid compared to 100% cross-reactivity with AKB48,
- wherein the IC50 values and cross-reactivity are determined by employing a tracer having the structure:
- wherein HRP is horseradish peroxidase.
5 more elements
A method of making an antibody comprising:
- administering an immunogen having the structure of:
- wherein KLH is keyhole limpet hemocyanin,
- to a mammal;
- and
- purifying the resultant antibody.
2 more elements
Dependencies are inferred from the claim text.