In vitro diagnosis device and uses thereof

A device, for detection of at least one reaction between an antigen of erythrocytic phenotype and an antibody directed specifically against this antigen, from a sample of blood or one of its components, comprising:

• (i) a support, and
• (ii) a hydrophobic porous membrane, wherein the membrane has a thickness between 0.05 mm and 1.5 mm, wherein the diameter of the pores is between 2 and 30 μm, said membrane comprising at least one hydrophilic reaction area for receiving said sample, wherein a surface area of the hydrophilic reaction area is less than a surface area of the hydrophobic porous membrane,
• wherein the support comprises a lower part on which is deposited the porous membrane and an upper part covering the membrane, said upper part comprises at least one opening, each opening corresponding to each hydrophilic reaction area of the porous membrane,
• wherein the hydrophilic reaction area of the porous membrane has been made hydrophilic with a detergent without modification of the chemical functions of the porous substrate by prior chemical or physical treatment of the porous membrane prior to the deposit of either the antigen or the antibody;
• and
• wherein the detergent is a solution selected from the group consisting of between 0.01% m/v and 5% m/v polysorbate 20 in ethanol and an aqueous solution between 0.01% m/v and 5% m/v t-octylphenoxypolyethoxyethanol.