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US 10,591,492 B2Granted
Assays to detect neurodegeneration
AssigneeJanssen Pharmaceutica NV
InventorsHartmuth Christian Kolb, Gallen Triana-Baltzer, John Randall Slemmon
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Abstract
Methods of measuring the amount of singly- or multiply-phosphorylated p217+ tau protein in a sample are provided. Methods of detecting or diagnosing tauopathies, methods of determining the effectiveness of a treatment of a tauopathy, and methods of determining whether a subject is suitable for anti-p217+ tau antibody therapy are also provided. Also described are antibodies for use in the methods and kits comprising the antibodies.
Claims23
A kit comprising:
- a. a capture antibody comprising an immunoglobulin heavy chain HCDR1, HCDR2 and HCDR3 comprising the polypeptide sequences of SEQ ID NOs:
- 32, 33 and 34, respectively and an immunoglobulin light chain LCDR1, LCDR2 and LCDR3 comprising the polypeptide sequences of SEQ ID NOs:
- 35, 36 and 37, respectively;
- and
- b. at least one detection antibody comprising
- i. an antibody comprising an immunoglobulin heavy chain HCDR1, HCDR2 and HCDR3 comprising the polypeptide sequences of SEQ ID NOs:
- 12, 13 and 14, respectively and an immunoglobulin light chain LCDR1, LCDR2 and LCDR3 comprising the polypeptide sequences of SEQ ID NOs:
- 15, 16 and 17, respectively or
- ii. antibody comprising an immunoglobulin heavy chain HCDR1, HCDR2 and HCDR3 comprising the polypeptide sequences of SEQ ID NOs:
- 2, 3 and 4, respectively and an immunoglobulin light chain LCDR1, LCDR2 and LCDR3 comprising the polypeptide sequences of SEQ ID NOs:
- 5, 6 and 7, respectively.
8 more elements
A method of measuring p217+ tau peptides in a sample, comprising:
- a) contacting the sample with a capture antibody comprising an immunoglobulin heavy chain HCDR1, HCDR2 and HCDR3 comprising the polypeptide sequences of SEQ ID NOs:
- 32, 33 and 34, respectively and an immunoglobulin light chain LCDR1, LCDR2 and LCDR3 comprising the polypeptide sequences of SEQ ID NOs:
- 35, 36 and 37, respectively to capture the p217+ tau peptides in the sample, and
- b) contacting the captured p217+ tau peptides from (a) with at least one of a detection antibody selected from
- i. an antibody comprising an immunoglobulin heavy chain HCDR1, HCDR2 and HCDR3 comprising the polypeptide sequences of SEQ ID NOs:
- 12, 13 and 14, respectively and an immunoglobulin light chain LCDR1, LCDR2 and LCDR3 comprising the polypeptide sequences of SEQ ID NOs:
- 15, 16 and 17, respectively and
- ii. antibody comprising an immunoglobulin heavy chain HCDR1, HCDR2 and HCDR3 comprising the polypeptide sequences of SEQ ID NOs:
- 2, 3 and 4, respectively and an immunoglobulin light chain LCDR1, LCDR2 and LCDR3 comprising the polypeptide sequences of SEQ ID NOs:
- 5, 6 and 7, respectively
- to thereby measure at least one of an amount of the p217+ tau peptides and an amount of long p217+ tau peptides, respectively comprising.
8 more elements
Dependencies are inferred from the claim text.