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US 10,591,490 B2Granted
Methods and kits for determining von willebrand factor activity in the absence of ristocetin
AssigneeSiemens Healthcare Diagnostics Products GmbH
InventorsHarald Althaus, Tobias Obser, Juergen Patzke, Reinhard Schneppenheim
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Abstract
Described herein are method(s), kit(s), reagent(s) and the like for determining von Willebrand factor (VWF) activity in a sample in the absence of ristocetin.
Claims20
A method for determining von Willebrand factor (VWF) activity in a biological sample from a human, wherein the sample is mixed with purified glycoprotein Ib heavy chain (GPIbα) protein and with a particulate solid phase to give an assay mix, the method comprising:
- providing an isolated human GPIbα protein or functional fragment thereof, wherein the isolated human GPIbα protein comprises mutations G233V and M239V relative to SEQ ID NO:1;
- providing the particulate solid phase which is capable of being associated with the isolated human GPIbα protein;
- wherein neither ristocetin nor botrocetin is added to the assay mix;
- and
- determining the VWF activity in the assay mix.
2 more elements
A method for determining von Willebrand factor (VWF) activity in a biological sample from a human, the method comprising:
- providing the biological sample;
- providing an assay mix by a process including:
- mixing the biological sample with an isolated human glycoprotein Ib heavy chain (GPIbα) protein or functional fragment thereof, wherein the isolated human GPIbα protein comprises mutations G233V and M239V relative to SEQ ID NO:1;
- and
- mixing the biological sample with a particulate solid phase capable of being associated with the isolated human GPIbα protein;
- wherein neither ristocetin nor botrocetin is added to the assay mix;
- and
- determining the VWF activity in the assay mix.
5 more elements
Dependencies are inferred from the claim text.