Methods and tools for detecting interactions in eukaryotic cells using microtubule structures and dynamics

A method for detecting an interaction between one or more protein bait and one or more candidate prey in an eukaryotic cell, comprising the steps of:

• a) providing an eukaryotic cell expressing (i) one or more protein bait, and (ii) one or more candidate prey, wherein said protein bait comprises a bait moiety and a polymerized tubulin-binding moiety, and wherein the one or more protein bait binds to polymerized tubulin within the eukaryotic cell via the polymerized tubulin-binding moiety, and
• b) detecting an interaction between said one or more protein bait and said one or more candidate prey in the eukaryotic cell by detecting the binding of the candidate prey to the bait moiety of the one or more protein bait localized along the polymerized tubulin.