Method for the detection of an IgM antibody specific for a flavivirus in a sample
AssigneeMedizinische Universität Wien
InventorsFranz X. Heinz, Karin Stiasny
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Abstract
Disclosed is a method for the detection of an IgM antibody specific for a flavivirus in a sample, comprising the steps of (a) contacting the sample with a solid support comprising immobilised IgM-binding molecules, (b) allowing binding of IgM antibodies in the sample to the IgM binding molecules on the solid support so that the IgM antibodies are also immobilised on the solid support, and (c) detecting IgM antibodies specific for a flavivirus by allowing binding of a complex comprising (i) an antiparallel dimer of soluble flavivirus Protein E (sE) and (ii) a marker and identifying the binding of the complex to the specific flavivirus IgM antibody by detecting the marker; and a kit suitable for performing the method.
Claims20
A method for the detection of an IgM antibody specific for a flavivirus in a sample, comprising:
- (a) contacting the sample with a solid support comprising immobilised IgM-binding molecules,
- (b) allowing binding of IgM antibodies in the sample to the IgM binding molecules on the solid support so that the IgM antibodies are also immobilised on the solid support, and
- (c) premixing an antiparallel dimer of soluble flavivirus Protein E (sE) with a marker for formation of a complex comprising the sE dimer and the marker, wherein the antiparallel dimer of sE comprises a tag and wherein the tag links the marker to the antiparallel dimer of sE, and
- (d) detecting IgM antibodies specific for a flavivirus by allowing binding of the preformed complex and identifying the binding of the complex to the specific flavivirus IgM antibody by detecting the marker.
Dependencies are inferred from the claim text.