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US 10,591,479 B2Granted
Detection of activated monocytes in human peripheral blood and determining the concentration of cell surface integrin using plasmon amplified raman detection
OwnerZehong Gao, Boer Liinventors
Life of this patent
Estimating the term…
Abstract
Cell surface Raman spectroscopy and a quantitative measure of cell surface integrin from monocytes isolated from peripheral blood provide a measure of active inflammation. Patients with elevated lipid profiles and inflammatory status are at high risk for plaque producing atherosclerosis and candidates for aggressive treatment and clinical follow-up.
Claims7
A method of determining monocyte activation comprising:
- a) providing a blood sample from an individual;
- b) isolating monocytes from the blood sample;
- c) depositing the isolated monocytes on a spectroscopic support so that the isolated monocytes adhere to the spectroscopic support;
- d) illuminating the adhered monocytes with near infra-red light;
- e) recording a first Raman spectrum of the adhered monocytes;
- and
- f) analyzing the Raman spectrum to detect biochemical transformations upon the monocyte cell membrane fluidity that render the isolated monocytes adhesive to the spectroscopic support, wherein the isolated activated monocytes are characterized as having decreased membranous lipid and reduced membrane integrity.
4 more elements
A method of determining monocyte activation comprising:
- a) providing a blood sample from an individual;
- b) isolating monocytes from the blood sample;
- c) depositing the isolated monocytes on a spectroscopic support so that the isolated monocytes adhere to the spectroscopic support;
- d) illuminating the adhered monocytes with near infra-red light;
- e) recording a first Raman spectrum of the adhered monocytes;
- f) binding anti-integrin-antibody-nanoparticles to the surface of the adhered monocytes;
- g) washing away unbound anti-integrin-antibody-nanoparticles;
- h) recording a second Raman spectrum;
- and
- i) comparing the intensity of the first Raman spectrum to the intensity of the second Raman spectrum, thereby measuring concentration of cell surface integrin bound to the monocytes.
7 more elements
A method of determining monocyte activation comprising:
- a) providing a blood sample from an individual;
- b) depositing monocytes in the blood sample on a spectroscopic support so that the isolated monocytes adhere to the spectroscopic support;
- c) binding anti-integrin-antibody-nanoparticles to the surface of the adhered monocytes;
- d) washing away unbound anti-integrin-antibody-nanoparticles;
- e) lysing the monocytes;
- f) recovering the bound anti-integrin-antibody-nanoparticles;
- g) generating a Raman spectrum of the bound anti-integrin-antibody-nanoparticles, thereby measuring concentration of cell surface integrin bound to the monocytes.
4 more elements
Dependencies are inferred from the claim text.