In vitro blood-brain barrier model
AssigneesInstitut Pasteur, Institut National de la Sante et de la Recherche Medicale
InventorsChristophe Prehaud, Monique Lafon, Pierre-Emmanuel Ceccaldi, Philippe Afonso, Pierre Lafaye
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Abstract
The invention is in the domain of delivery of molecules to brain cells across the blood-brain barrier. The invention relates to a novel polypeptide-based carrier that allows the efficient delivery of an effector peptide, to neuron cells across the blood-brain barrier, and to methods for the production and testing of such carrier, including a model for testing the capacity of such molecule to cross the blood-brain barrier and/or the toxicity of molecules on the blood brain barrier and/or the capacity of molecules that have crossed to target human brain cells (e/g. neurons, astrocytes and microglial cells).
Claims18
An in vitro cell culture device comprising a container comprising a filter positioned in such a manner that it separates the container into a first compartment and a second compartment, wherein the filter comprises at least one confluent monolayer of immortalized human endothelial cells on a surface of the filter facing the first compartment and wherein the second compartment does not contain immortalized human endothelial cells and does contain immortalized human microglial cells to thereby provide a functional blood brain barrier model.
A method of preparing a cell culture device the method comprising:
- (i) providing a filter carrying at least one confluent monolayer of immortalized human endothelial cells on a surface of the filter in a container,
- (ii) positioning the filter so that it separates the container into a first compartment and a second compartment, wherein the confluent monolayer of immortalized human endothelial cells on the surface of the filter are facing the first compartment, and
- (iii) providing immortalized human microglial cells in a second compartment.
Dependencies are inferred from the claim text.