Systems and methods for determining sample osmolarity
AssigneeLacrisciences, LLC
InventorsPaul T. Gavaris, Chris D. Geddes, Ali H. Ghovanlou
Life of this patent
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Abstract
Systems and methods for determining the osmolarity of a sample are provided. Aspects of the subject methods include contacting a sensing surface of a surface plasmon resonance based sensor with a sample, and generating one or more data sets at at least two wavelengths over a time interval, wherein the data sets are used to determine the osmolarity of the sample. The subject methods find use in determining the osmolarity of a sample, such as a biological sample (e.g., a tear fluid), and in the diagnosis and/or monitoring of various diseases and disorders, such as, e.g., dry eye disease.
Claims20
A system comprising:
- (i) a sensor comprising a sensing surface comprising a coated region, wherein the sensor is configured to:
- direct a first optical signal to interact with the sensing surface over a first range of incident angles;
- and
- direct a second optical signal to interact with the sensing surface over a second range of incident angles,
- wherein the first range of incident angles is different from the second range of incident angles;
- and
- (ii) an optical chassis comprising:
- an optical signal generating component;
- a detection component;
- a processor;
- a controller;
- and
- a non-transitory computer-readable medium comprising instructions that, when executed by the processor, cause the controller to:
- direct an optical signal having a first wavelength to interact with the sensing surface over the first range of incident angles to generate a first surface plasmon resonance (SPR) signal;
- generate a series of images of the first SPR signal over a first time interval using the detection component;
- determine a series of pixel positions that correspond to a minimum value of the first SPR signal over the first time interval;
- direct an optical signal having a second wavelength to interact with the sensing surface over the first range of incident angles to generate a second SPR signal;
- generate a series of images of the second SPR signal over a second time interval using the detection component;
- determine a series of pixel positions that correspond to a minimum value of the second SPR signal over the second time interval;
- compare the series of pixel positions that correspond to the minimum value of the first SPR signal over the first time interval to the pixel position of at least one reference feature to generate a first reference-corrected SPR function;
- compare the series of pixel positions that correspond to the minimum value of the second SPR signal over the second time interval to the pixel position of the at least one reference feature to generate a second reference-corrected SPR function;
- and
- compare one or more characteristics of the first reference-corrected SPR function and the second reference-corrected SPR function to determine a reference-corrected SPR delta pixel value.
Dependencies are inferred from the claim text.