Cell capture system and method of use
AssigneeCelsee Diagnostics, Inc.
InventorKalyan Handique
Life of this patent
Estimating the term…
Abstract
A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.
Claims20
A method for automatically processing target cellular material from cells separated in single-cell format, the method comprising:
- at a workstation, receiving a substrate comprising a network having an input channel at an upstream end, one or more output channels at a downstream end, and a set of microfluidic elements in communication with the input channel and the one or more output channels, wherein flow from the input channel is configured to reach at least one of the one or more output channels only by way of the set of microfluidic elements;
- at the workstation, positioning a container comprising a set of target particles;
- from a controller in communication with a handling subsystem of the workstation, receiving a set of instructions for automatic processing of the set of target particles, in single-particle format;
- and
- based upon the set of instructions:
- with the handling subsystem, transmitting the set of target particles into the network of the substrate;
- and
- isolating each of the set of target particles in single-particle format with an isolation material, within the network of the substrate.
A system for automatically processing target cellular material, the system comprising:
- a workstation configured to accept and position a substrate comprising a network having an input channel at an upstream end, one or more output channels at a downstream end, and a set of microfluidic elements in communication with the input channel and the one or more output channels, wherein flow from the input channel is configured to reach at least one of the one or more output channels only by way of the set of microfluidic elements, the workstation further comprising:
- a handling subsystem comprising a pump;
- a thermal control substation comprising a heater;
- a magnet;
- a barcode reader;
- and
- a controller configured to execute a set of instructions for automatic processing of a set of target particles, in single-particle format, the set of instructions for performing:
- with the handling subsystem, transmitting the set of target particles from a container into the network of the substrate;
- isolating each of the set of target particles in single-particle format with an isolation material, within the network of the substrate, by way of the pump of the handling subsystem;
- and
- establishing thermal communication between cell-derived material from the set of target particles and the heater.
Dependencies are inferred from the claim text.