Multiplexed analysis of cell-materials in niches
AssigneeGeorgia Tech Research Corporation
InventorsKrishnendu Roy, Kirsten Parratt
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Abstract
Embodiments of the present disclosure can comprise a method for multiplexed analysis. The method can comprise acquiring interrogation data associated with a microstructure in a population and analyzing the microstructure based on the interrogation data. In some embodiments, the microstructure can have a different shape than at least another microstructure in the population and comprise a plurality of cells. Additionally, the acquiring the interrogation data can comprise acquiring interrogation data of microstructures in a population at a structure concentration of at least 100 microstructures/μL. Therefore, in some embodiments, acquiring the interrogation data can comprises flowing the population through a flow cytometer.
Claims19
A method for multiplexed analysis comprising:
- acquiring interrogation data associated with encapsulating microstructures in a population at a structure concentration of at least 100 microstructures/μL;
- identifying from the interrogation data at least two multiplexing parameters of at least a portion of the encapsulating microstructures, wherein one of the at least two multiplexing parameters comprises the shape of the encapsulating microstructures, and another of the at least two multiplexing parameters selected from the group consisting of size and fluorescence of the encapsulating microstructures;
- and
- indexing at least a portion of the encapsulating microstructures based on a pre-determined barcoding system;
- wherein at least one encapsulating microstructure has a different shape than another encapsulating microstructure in the population;
- and
- wherein at least a portion of the encapsulating microstructures each comprise a hydrogel with suspensions selected from the group consisting of one or more cells, one or more analyte sensing capture agents, and combinations thereof.
A method for multiplexed analysis comprising:
- non-destructively acquiring interrogation data associated with a population at a structure concentration of at least 100 microstructures/μL, the population comprising microstructures, each microstructure comprising:
- a three-dimensional microgel niche;
- and
- one or more multiplexing parameters of the microstructures selected from the group consisting of shape, size and fluorescence;
- identifying from the interrogation data at least two multiplexing parameters of at least a portion of the microstructures, one comprising the shape of the microstructures, and another of the at least two multiplexing parameters selected from the group consisting of size and fluorescence of the microstructures;
- indexing at least a portion of the microstructures based on a pre-determined barcoding system comprising barcodes, each barcode different from one another, and each barcode based at least in part on at least one of the multiplexing parameters selected from the group consisting of size and fluorescence of the microstructures;
- and
- sorting at least a portion of the of the microstructure population based on the indexing;
- wherein at least one microstructure has a different shape than at least another microstructure.
Dependencies are inferred from the claim text.