Equipment for the chromatic discrimination and counting of organisms
AssigneeUniversidade De Aveiro
InventorsMiguel Augusto Mendes Oliveira E Silva, Rui Manuel Escadas Ramos Martins, Amadeu Mortágua Velho Da Maia Soares, Sizenando Nogueira De Abreu
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Abstract
The present disclosure relates to a device for chromatic discrimination and counting of organisms in a liquid medium with application to organisms belonging to the genus Daphnia, Ceriodaphnia, Artemia, as well Collembola; or similar organisms. The device can be applied in the field of ecotoxicology and involves technologies in the areas of analytical instrumentation, electronics, computer science and biology.
Claims8
A device for chromatic discrimination and counting of organisms in liquid medium sample, comprising:
- a channel for conducting the sample, wherein the channel has an inner section having an internal diameter sized to pass no more than one organism through the inner section at a time so that all the organisms are driven sequentially one-by-one through said channel wherein said channel is a tubular stylet;
- a collector for conducting the sample to that channel wherein said collector is a funnel;
- two or more light emitters to emit a chromatic signal to that channel;
- two or more light receivers to capture the chromatic signal of the organism intercepting that channel;
- wherein light emitters and receivers are radially distributed around an optical window located in a cross section of the stylet;
- wherein the chromatic signal present in the channel is a chromatic signal absorbed, reflected, refracted and/or self-issued by the organisms;
- wherein a first light receiver of the two or more light receivers is at an angle of 180° from a second light emitter of the two or more light emitters to capture the chromatic signal absorbance of the organism;
- wherein further of the two or more light receivers are placed at an angle less than 180° from the first light emitter to capture the chromatic signal reflection, refraction and/or the self-emission of the organism;
- an electronic data processor configured to differentiate the organisms counted according to the chromatic signal received;
- wherein the organisms are neonates of Daphnia, Ceriodaphnia, and Artemia, fish eggs, and similar sized organisms.
Dependencies are inferred from the claim text.