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US 10,590,489 B2Granted
Detecting neoplasm
AssigneeMayo Foundation for Medical Education and Research
InventorsWilliam R. Taylor, Jonathan J. Harrington, Patrick S. Quint, Hongzhi Zou, Harold R. Bergen, III, David I. Smith, David A. Ahlquist
Life of this patent
Estimating the term…
Abstract
This document relates to methods and materials for detecting premalignant and malignant neoplasms. For example, methods and materials for determining whether or not a stool sample from a mammal contains nucleic acid markers or polypeptide markers of a neoplasm are provided.
Claims2
A method, comprising:
- (a) extracting genomic DNA from a biological sample of a human individual suspected of having precancer or colorectal cancer, wherein the biological sample is a stool sample;
- (b) amplifying a portion of the extracted genomic DNA with primers specific for K-ras, and
- measuring a K-ras mutation score by performing a digital melt curve analysis or quantitative allele-specific PCR;
- and
- (c) treating a portion of the extracted genomic DNA with bisulfite;
- amplifying the bisulfite-treated genomic DNA with primers specific for BMP3;
- and
- measuring the methylation level of one or more CpG sites in BMP3 by methylation-specific PCR, quantitative methylation-specific PCR, methylation-sensitive DNA restriction enzyme analysis, quantitative bisulfite pyrosequencing, or bisulfite genomic sequencing PCR.
5 more elements
Dependencies are inferred from the claim text.