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US 10,590,484 B2Granted
Methods and compositions for sequencing modified nucleic acids
AssigneePacific Biosciences of California, Inc.
InventorsJonas Korlach, Stephen Turner, Tyson A. Clark
Life of this patent
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Abstract
Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.
Claims13
A method of determining whether a cell sample comprises actively dividing cells, the method comprising:
- obtaining a non-amplified, double-stranded genomic DNA library from the cell sample;
- performing single-molecule sequencing to generate sequence reads for both strands of a plurality of individual double-stranded nucleic acids in the genomic DNA library, wherein the sequence reads comprise both base sequence data and base modification data;
- and
- analyzing the base sequence and base modification of the plurality of individual double-stranded nucleic acids, wherein identification of individual double-stranded nucleic acids in the genomic DNA library that are hemi-modified indicates that the cell sample comprises actively dividing cells.
1 more element
Dependencies are inferred from the claim text.