Detection of immune cells, in particular T cells through DNA-methylation analysis of the genes CCR6 and BLR1
AssigneeEpiontis GmbH
InventorSven Olek
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Abstract
The present invention relates to a method, in particular an in vitro method, for identifying certain immune cells of a mammal, comprising analysing the methylation status of at least one CpG position in the gene CCR6 and/or BLR1 or an orthologous or paralogous gene thereof, and the use of DNA-methylation analysis of the genes of the proteins CCR6 and/or BLR1 for a detection and quality assurance and control of certain immune cells. In particular, the present invention relates to analysing the methylation status of at least one CpG position in the gene CCR6 in T cells. Furthermore, the present invention relates to a kit for performing the above methods, as well as to respective uses.
Claims12
A method for identifying immune cells in a sample from a mammal wherein said method comprises:
- (a) amplifying the BLR1 gene or an orthologous or paralogous gene thereof with a primer pair comprising SEQ ID NO:
- 9 and SEQ ID NO:
- 10, or orthologous or paralogous primer pairs thereof to generate an amplicon;
- and
- (b) analyzing the amplicon from step (a) for a methylation status of at least one CpG at position 23, 25, 46, 51, 109, 157, 177, 183, 199, 229, 244, 247, 287, or 360 or an equivalent CpG position in the amplicon of a BLR1 orthologous or paralogous gene,
- wherein demethylation at the at least one CpG position is indicative of the immune cells.
Dependencies are inferred from the claim text.