Target enrichment by single probe primer extension

A method of amplifying a target sequence comprising the steps of:

• a) contacting the target nucleic acid with a primer and a polymerase, wherein the primer comprises a target-binding site and a unique molecular identification tag (UID);
• b) conducting a polymerase extension step comprising incorporation of uridine-containing bases into the primer comprising the UID and performing a strand cleavage step comprising treatment of the primer extension product with uracil-N-DNA glycosylase and cleavage at the abasic sites of the primer extension product;
• c) ligating adaptors to each end of a single-stranded primer extension product comprising the UID, to create a ligation product, wherein adaptors comprise at least one universal priming site;
• d) amplifying the ligation product in an amplification reaction utilizing at least one primer binding to the at least one universal priming site to create the amplified target sequence.