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US 10,590,470 B2Granted
Methods and systems for performing digital measurements
AssigneeUniversity of Washington through its Center for Commercialization
InventorsDaniel T. Chiu, Bryant S. Fujimoto, Alexander R. Gansen, Gloria S. Yen, Robert M. Lorenz
Life of this patent
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Abstract
Methods, devices and systems for performing digital measurements are provided.
Claims27
A method for using digital measurements to determine a concentration of an analyte in a sample, the method comprising:
- producing a plurality of droplets, wherein at least one of the droplets of the plurality of droplets comprises the analyte from the sample and a detectable agent;
- measuring some or all of the droplets of the plurality of droplets to determine a volume of the some or all of the droplets of the plurality of droplets;
- analyzing at least a portion of the plurality of droplets to determine a number of droplets in the at least a portion of the plurality of droplets that contain the analyte and the detectable agent;
- and
- using the volume of the some or all of the plurality of droplets and the number of droplets in the at least a portion of the plurality of droplets that contain the analyte and the detectable agent to determine the concentration of the analyte in the sample.
2 more elements
A system for using digital measurements to determine a concentration of an analyte in a sample, the system comprising:
- a sample holder containing a plurality of droplets;
- a detector for detecting a detectable agent contained in at least one droplet of the plurality of droplets, the at least one droplet additionally comprising the analyte;
- and
- a computer comprising a memory device with executable instructions stored thereon, the instructions, when executed by a processor, cause the processor to:
- measure some or all of the droplets of the plurality of droplets to determine a volume of the some or all of the droplets of the plurality of droplets;
- analyze at least a portion of the plurality of droplets to determine a number of droplets in the at least a portion of the plurality of droplets that contain the analyte and the detectable agent;
- and
- use the volume of the some or all of the plurality of droplets and the number of droplets in the at least a portion of the plurality of droplets that contain the analyte and the detectable agent to determine the concentration of the analyte in the sample.
5 more elements
Dependencies are inferred from the claim text.