Methods and kits for analyzing DNA binding moieties attached to DNA
AssigneeYeda Research and Development Co. Ltd.
InventorsIdo Amit, David Lara-Astiaso, Meital Gury
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Abstract
A method of analyzing DNA which, in a cell, is bound to a DNA binding moiety, the method comprising: (a) obtaining at least two samples of complexes of DNA bound to a DNA binding moiety; (b) isolating the complexes on a solid support; (c) labeling the DNA of the complexes, wherein the labeling distinguishes between the complexes of the first of the at least two samples and the complexes of the second of the at least two samples; (d) pooling the at least two samples of complexes; and (e) isolating the complexes using an agent which specifically binds to the DNA binding moiety; and (f) analyzing the DNA of the complexes.
Claims14
A method of analyzing DNA which, in a cell, is bound to a DNA binding moiety, the method comprising:
- (a) obtaining at least two samples of complexes of DNA bound to a DNA binding moiety, wherein said DNA is derived from a cell sample comprising 100-10,000 cells;
- (b) crosslinking said DNA to said DNA binding moiety;
- (c) isolating said complexes on a solid support;
- (d) labeling the DNA of said complexes, wherein said labeling distinguishes between said complexes of the first of said at least two samples and said complexes of the second of said at least two samples;
- (e) pooling said at least two samples of complexes;
- and
- (f) isolating said complexes using an agent which specifically binds to said DNA binding moiety;
- and
- (g) analyzing said DNA of said complexes.
Dependencies are inferred from the claim text.