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US 10,590,466 B2Granted
Plant based dye for staining of biological samples, extraction method and uses thereof
AssigneeFathima Benazir Jahangir Ali
InventorsFathima Benazir Jahangir Ali, Alex Dass Paul Rabidass, Handanahal Subbarao Savithri
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Abstract
The present disclosure provides a composition comprising brazilein which can be used as a fluorescent dye for enhanced visualization of nucleic acids such as DNA, and RNA. Also provided in the specification is a simple, time and cost effective method of extraction of brazilein from bark starting material.
Claims14
A composition for detection of nucleic acids comprising:
- a. brazilein((6aS,11bR)-7,11b-Dihydro-6H-indeno[2,1-c]chromene-3,6a,9,10-tetrol);
- b. at least one solvent selected from the group consisting of methanol, ethanol, acetone, and combinations thereof;
- and
- c. at least one compound selected from the group consisting of glycerol, sodium chloride, magnesium sulfate, dimethyl sulfoxide, and combinations thereof.
1 more element
Dependencies are inferred from the claim text.