Selective enrichment medium for carbapenem-resistant bacteria
OwnerAlain Rambachinventor
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Abstract
The present invention relates to a method for direct detection and differentiation of carbapenem-resistant bacteria in a sample comprising (i) inoculation with said sample of a culture medium comprising at least meropenem and/or ertapenem and at least one chromogenic agent, (ii) incubation of said culture medium under conditions allowing the growth of carbapenem-resistant bacteria, and (iii) detection of colonies formed on said culture medium corresponding to a carbapenem-resistant bacteria, as well as a culture medium suitable for use in such a method.
Claims7
A method for direct detection of carbapenem-resistant bacteria in a sample comprising the following successive steps:
- a) Inoculating a culture medium with said sample, wherein said culture medium comprises a sole antibiotic, said sole antibiotic being ertapenem, and at least one chromogenic agent,
- b) Incubation of said culture medium under conditions allowing the growth of carbapenem-resistant bacteria, and
- c) Detection of colonies formed on said culture medium corresponding to carbapenem-resistant bacteria,
- wherein said chromogenic agent is hydrolyzed into a chromophore selected from a group consisting of O-nitrophenyl, P-nitrophenyl, Chloronitrophenyl, Hydroxyphenyl, Nitroanilide, Phenolphthalein, Thymophthalein, Hydroxyquinoline, Cyclohexenoesculetine, Dihydroxyflavone, Catechol, Resazurin, Resofurin, VBzTM, VLM, VLPr, VQM, Indoxyl, 5-bromo-4-chloro-3-indoxyl, 5-bromo-6-chloro-3-indoxyl, 6-chloro-3-indoxyl, 6-fluoro-3-indoxyl, 5-iodo-3-indoxyl, and N-methylindoxyl, wherein said method does not include a preliminary isolation step of the different bacterial strains present in the sample.
Dependencies are inferred from the claim text.