Loading patent…
AssigneesNorthwestern University, The Board of Trustees of the University of Illinois
InventorsMichael Christopher Jewett, Do Soon Kim, Erik D. Carlson, Alexander S. Mankin, Cedric Orelle, Teresa Szal, Anne E. d'Aquino
Estimating the term…
An engineered ribosome that includes a tethered subunit arrangement is disclosed, in which the engineered ribosome supports translation of a sequence defined polymer. Methods for making and using the engineered ribosome are also disclosed, including a method for preparing a sequence defined polymer using the engineered ribosome and a method for preparing a sequence defined polymer using the engineered ribosome in a two-protein translation system. The engineered ribosomes may be utilized in methods for incorporating unnatural amino acids into a sequence defined polymer. Also disclosed are optimized polynucleotide sequences for use as tethers and Shine-Dalgarno/anti-Shine-Dalgarno sequences.
An engineered ribosome, the engineered ribosome comprising a small subunit, a large subunit, and a linking moiety, wherein the small subunit comprises ribosomal RNA (rRNA), wherein the large subunit comprises rRNA, and wherein the linking moiety tethers the rRNA of the small subunit with the rRNA of the large subunit, wherein the linking moiety comprises a polynucleotide sequence.
The engineered ribosome of 10, wherein the ribosome comprises the S1 polynucleotide domain followed, in order, by the T1 polynucleotide domain, the L1 polynucleotide domain, the C polynucleotide domain, the L2 polynucleotide domain, the T2 polynucleotide domain, and the S2 polynucleotide domain, from 5′ to 3′.
Dependencies are inferred from the claim text.