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US 10,590,448 B2Granted
Production of galacto-oligosaccharides
AssigneeFriesland Campina Nederland B.V.
InventorsFrédéric Benjamins, Linqiu Cao, Antonius Augustinus Broekhuis
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Abstract
The invention relates to the enzymatic preparation of galacto-oligosaccharides (GOS). Provided is a method for preparing GOS from lactose, comprising (i) contacting a lactose feed with immobilized beta-galactosidase (EC 3.2.1.23) and (ii) allowing for GOS synthesis, wherein said lactose feed is an aqueous slurry of crystalline lactose.
Claims14
A method for preparing galacto-oligosaccharides (GOS) from lactose, comprising
- (i) dissolving lactose crystals in an aqueous phase at room temperature to provide an aqueous slurry of crystallized lactose that contains at least 53% (w/w) lactose,
- (ii) heating said aqueous slurry of crystallized lactose from room temperature to a desired reaction temperature of between 20° C.-60° C.;
- (iii) contacting said heated aqueous slurry of crystallized lactose with Bacillus circulans beta-galactosidase immobilized on a porous carrier;
- and
- (iv) allowing for GOS synthesis, thereby preparing GOS from lactose.
2 more elements
Dependencies are inferred from the claim text.