Adeno-associated virus (AAV) serotype 8 sequences, vectors containing same, and uses therefor
AssigneeThe Trustees of the University of Pennsylvania
InventorsGuangping Gao, James M. Wilson, Mauricio R. Alvira
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Abstract
Sequences of a serotype 8 adeno-associated virus and vectors and host cells containing these sequences are provided. Also described are methods of using such host cells and vectors in production of rAAV particles.
Claims25
A cultured host cell containing a recombinant nucleic acid molecule encoding an AAV vp1 capsid protein having a sequence comprising amino acids 1 to 738 of SEQ ID NO:
- 2 (AAV8) or a sequence at least 95% identical to the full length of amino acids 1 to 738 of SEQ ID NO:
- 2, wherein the recombinant nucleic acid molecule further comprises a heterologous non-AAV sequence.
A cultured host cell containing a recombinant nucleic acid molecule encoding an AAV vp2 capsid protein having a sequence comprising amino acids 138 to 738 of SEQ ID NO:
- 2 (AAV8) or a sequence at least 95% identical to the full length of amino acids 138 to 738 of SEQ ID NO:
- 2, wherein the recombinant nucleic acid molecule further comprises a heterologous non-AAV sequence.
A cultured host cell containing a recombinant nucleic acid molecule encoding an AAV vp3 capsid protein having a sequence comprising amino acids 204 to 738 of SEQ ID NO:
- 2 (AAV8) or a sequence at least 95% identical to the full length of amino acids 204 to 738 of SEQ ID NO:
- 2, wherein the recombinant nucleic acid molecule further comprises a heterologous non-AAV sequence.
A cultured host cell containing a recombinant nucleic acid molecule comprising
- (a) nucleotides 2121 to 4334 of SEQ ID NO:
- 1 or a sequence at least 95% identical to nucleotides 2121 to 4334 of SEQ ID NO:
- 1,
- (b) nucleotides 2532 to 4334 of SEQ ID NO:
- 1 or a sequence at least 95% identical to nucleotides 2532 to 4334 of SEQ ID NO:
- 1, or
- (c) nucleotides 2730 to 4334 of SEQ ID NO:
- 1 or a sequence at least 95% identical to nucleotides 2730 to 4334 of SEQ ID NO:
- 1,
- wherein the recombinant nucleic acid molecule further comprises a heterologous non-AAV sequence.
Dependencies are inferred from the claim text.