US 10,590,434 B2 · Highly inducible dual-promoter lentiviral TET-ON system

Abstract

The invention relates to expression systems useful for regulated expression of a gene of interest based on the constitutive expression of the original TetR repressor and the expression of the polynucleotide driven by a constitutive promoter operably linked to an operator sequence for a tetracycline operator sequence. The system can be provided as two different polynucleotides or as an all-in-one vector. The invention also relates to vectors, host cells and viral particles according to the invention as well as to the uses thereof for in vitro and in vivo production of products of interest or for therapy.

Claims16

Dependent claims

A polynucleotide comprising

(i) a transcriptional regulatory sequence comprising a first promoter and at least one binding site for a transcriptional repressor wherein said first promoter and said at least one binding site for a transcriptional repressor are arranged so that the binding of a transcriptional repressor to said at least one binding site inhibits a transcriptional activity of the promoter and
(ii) an expression cassette comprising a polynucleotide encoding a regulatable transcriptional repressor under the operative control of a second promoter wherein said regulatable transcriptional repressor is capable of specifically binding to the at least one binding site in the transcriptional regulatory sequence in the absence but not in the presence of a ligand thereof
wherein the second promoter is an elongation factor 1α (EF1α) promoter, and wherein the first promoter is a cytomegalovirus (CMV) intermediate early promoter.

Dependencies are inferred from the claim text.

Highly inducible dual-promoter lentiviral TET-ON system

Life of this patent

Highly inducible dual-promoter lentiviral TET-ON system

Life of this patent

Abstract

Claims16