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US 10,590,429 B2Granted
Targeted mutagenesis of tobacco berberine bridge enzyme-like nucleic acids
AssigneeNorth Carolina State University
InventorsRalph E. Dewey, Ramsey S. Lewis
Life of this patent
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Abstract
The present invention relates to modifications of berberine bridge enzyme-like nucleic acids and their use in modulation of nicotine biosynthesis in plants.
Claims23
A tobacco product comprising tobacco from a Nicotiana plant, wherein the plant:
- (A) is modified so as to reduce the activity of at least one of BBLa, BBLb, and BBLc or to reduce expression of a nucleic acid encoding at least one of BBLa, BBLb, and BBLc;
- and
- (B) comprises a mutation in at least one endogenous polynucleotide or combination of polynucleotides selected from the group consisting of:
- (i) a polynucleotide having about 97% to about 100% sequence identity to SEQ ID NO:1 and a polynucleotide having about 97% to about 100% sequence identity to SEQ ID NO:
- 2;
- (ii) a polynucleotide having about 97% to about 100% sequence identity to SEQ ID NO:
- 1 and a polynucleotide having about 97% to about 100% sequence identity to SEQ ID NO:
- 3;
- (iii) a polynucleotide having about 97% to about 100% sequence identity to SEQ ID NO:2;
- and
- (iv) a polynucleotide having about 97% to about 100% sequence identity to SEQ ID NO:3,
- such that the Nicotiana plant has a nicotinic alkaloid content that is reduced as compared to a plant that is not modified per (A) and does not comprise the mutations of (B).
9 more elements
A method of producing a Nicotiana plant having reduced nicotinic alkaloid content, comprising combining in a Nicotiana plant:
- (A) a modification that reduces the activity of at least one of BBLa, BBLb, and BBLc or reduces expression of a nucleic acid encoding at least one of BBLa, BBLb, and BBLc;
- and
- (B) a mutation in at least one endogenous polynucleotide or combination of polynucleotides selected from the group consisting of:
- (i) a polynucleotide having about 97% to about 100% sequence identity to SEQ ID NO:1 and, a polynucleotide having about 97% to about 100% sequence identity to SEQ 1D NO:
- 2;
- (ii) a polynucleotide having about 97% to about 100% sequence identity to SEQ ID NO:
- 1 and a polynucleotide having about 97% to about 100% sequence identity to SEQ ID NO:
- 3;
- (iii) a polynucleotide having about 97% to about 100% sequence identity to SEQ ID NO:2;
- and
- (iv) polynucleotide having about 97% to about 100% sequence identity to SEQ ID NO:3,
- such that the Nicotiana plant has a nicotinic alkaloid content that is reduced as compared to a plant that is not modified per (A) and does not comprise the mutations of (B).
9 more elements
Dependencies are inferred from the claim text.