RNA ligand-displaying exosomes for specific delivery of therapeutics to cell by RNA nanotechnology
AssigneeOhio State Innovation Foundation
InventorsPeixuan Guo, Fengmei Pi, Hui Li, Shaoying Wang
Life of this patent
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Abstract
Disclosed herein are compositions comprising extracellular vesicles, such as exosomes, displaying an RNA nanoparticle on its surface. The RNA nanoparticle can target the extracellular vesicle to a given cell via a targeting moiety. The extracellular vesicle can also comprise a functional moiety, which can be used in treatment or diagnostics.
Claims18
A composition comprising an RNA nanoparticle anchored on the surface of an extracellular vesicle membrane,
- wherein the nanoparticle is assembled from one or more ribonucleic acid strands duplexed together to form a secondary structure with three or more projecting stem loops,
- wherein at one of the three or more projecting stem loops is conjugated to a cholesterol moiety
- wherein at one of the three or more projecting stem loops comprises one or more functional moieties, and
- wherein at least one of the three or more projecting stem loops physically blocks encapsulation of the nanoparticle into the extracellular vesicle.
A method of targeting an extracellular vesicle to a cell of interest comprising:
- contacting the cell with a composition comprising an extracellular vesicle displaying an RNA nanoparticle on its surface,
- wherein the nanoparticle is assembled from one or more ribonucleic acid strands duplexed together to form a secondary structure with three or more projecting stem loops,
- wherein at one of the three or more projecting stem loops is conjugated to a cholesterol moiety,
- wherein at least one of the three or more projecting stem loops physically blocks encapsulation of the nanoparticle into the extracellular vesicle, and
- wherein at one of the three or more projecting stem loops comprises at least one targeting moiety that directs the extracellular vesicle to the cell of interest.
A method of treating disease in a subject, comprising administering to the subject composition comprising an extracellular vesicle displaying an RNA nanoparticle on its surface,
- wherein the nanoparticle is assembled from one or more ribonucleic acid strands duplexed together to form a secondary structure with three or more projecting stem loops,
- wherein at one of the three or more projecting stem loops is conjugated to a cholesterol moiety,
- wherein at least one of the three or more projecting stem loops physically blocks encapsulation of the nanoparticle into the extracellular vesicle, and
- wherein at one of the three or more projecting stem loops comprises one or more functional moieties capable of treating the disease in the subject.
A method of imaging a cell, the method comprising contacting the cell with a composition comprising an extracellular vesicle displaying an RNA nanoparticle on its surface,
- wherein the nanoparticle is assembled from one or more ribonucleic acid strands duplexed together to form a secondary structure with three or more projecting stem loops,
- wherein at one of the three or more projecting stem loops is conjugated to a cholesterol moiety
- wherein at least one of the three or more projecting stem loops physically blocks encapsulation of the nanoparticle into the extracellular vesicle, and
- wherein at one of the three or more projecting stem loops comprises one or more diagnostic moieties.
Dependencies are inferred from the claim text.