Asx-specific protein ligase
AssigneeNanyang Technological University
InventorsKien Truc Giang Nguyen, James P. Tam
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Abstract
The present invention is directed to enzymes having Asx-specific ligase and cyclase activity and to nucleic acids encoding those as well as methods of the manufacture of said enzymes. Further encompassed are methods and uses of these enzymes.
Claims13
A method for cyclizing a peptide or ligating peptides or proteins, the method comprising incubating said peptide or peptides or proteins with an isolated polypeptide having protein ligase or cyclase activity and comprising the amino acid sequence as set forth in SEQ ID NO:1 under conditions that allow cyclization of said peptide or ligation of said peptides or proteins, wherein the peptide to be cyclized comprises
- (i) the C-terminal, amino acid sequence (X)oN/D(X)p, wherein X is any amino acid and o and p are independently from each other integers of at least 2;
- or
- (ii) the C-terminal amino acid sequence (X)oN*/D*, wherein X is any amino acid, o is an integer of at least 2 and the C-terminal N/D residue is amidated in that the C-terminal carboxy group is replaced by an amide group of the formula —C(O)—N(R′)2, with R′ being any residue.
Dependencies are inferred from the claim text.