Recombinant microorganisms exhibiting increased flux through a fermentation pathway
AssigneeLanzatech New Zealand Limited
InventorsMichael Koepke, Alexander Paul Mueller, Loan Phuong Tran
Life of this patent
Estimating the term…
Abstract
The invention provides a recombinant, carboxydotrophic Clostridium bacterium that expresses one or more of pyruvate:ferredoxin oxidoreductase (EC 1.2.7.1), acetolactate synthase (EC 2.2.1.6), and acetolactate decarboxylase (EC 4.1.1.5). The invention further provides a method of producing a fermentation product by fermenting the recombinant bacterium in the presence of a gaseous substrate comprising CO to produce one or more of ethanol, butanol, isopropanol, isobutanol, higher alcohols, butanediol, 2,3-butanediol, succinate, isoprenoids, fatty acids, biopolymers, and mixtures thereof.
Claims16
A recombinant bacterium comprising a pyruvate:ferredoxin oxidoreductase (EC 1.2.7.1), an acetolactate synthase (EC 2.2.1.6), and an acetolactate decarboxylase (EC 4.1.1.5), wherein each enzyme is an overexpressed endogenous enzyme or an exogenous enzyme, wherein the bacterium is derived from Clostridium autoethanogenum, Clostridium ljungdahlii, or Clostridium ragsdalei, and wherein the bacterium produces 2,3-butanediol and acetate.
Dependencies are inferred from the claim text.