Amylase variants having reduced disposition to form deamidation products
AssigneeBASF SE
InventorsStefan Jenewein, Stefan Niedermaier
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Abstract
The present invention pertains to the field of amylases with reduced disposition to form deamidation products, particularly alpha-amylases, and compositions comprising such amylases. Also described are means and methods for producing such amylases, and uses of amylases and amylase-comprising compositions, for example for cleaning starch-containing stains, textile desizing, starch liquefaction and saccharification, baking and brewing. The invention also discloses means for increasing amylase purity during fermentation, formulation and storage of amylase or amylase-containing formulations.
Claims13
An amylase comprising an amino acid sequence having a sequence identity of at least 90% to SEQ ID NO. 1, wherein the amylase comprises substitutions at positions 3 and 314 in the numbering according to SEQ ID NO. 1,
- wherein each substitution is independently selected from any of A, D, E, F, G, H, I, K, L, P, R, S, T, V, W, and Y, and
- wherein the amino acid sequence having a sequence identity of at least 90% to SEQ ID NO. 1 imparts amylase activity to the amylase.
An enzyme composition comprising one or more amylases, wherein the molar ratio of amylases comprising any of L-isoAsp and D-isoAsp to amylases not comprising any of L-isoAsp and D-isoAsp is
- at most 1:1,
- and wherein the ratio is determined after 24 h of storage in aqueous 20 mM phosphate buffer at pH 8 and 37° C.
Dependencies are inferred from the claim text.