Esterases and uses thereof
AssigneeCarbios
InventorsVincent Tournier, Helene Texier, Marie-Laure Desrousseaux, Christopher Topham, Isabelle Andre, Sophie Barbe, Sophie Duquesne, Alain Marty
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Abstract
The present invention relates to novel esterase, more particularly to esterase variants having improved activity compared to the esterase of SEQ ID NO: 1 and the uses thereof for degrading polyester containing material, such as plastic products. The esterases of the invention are particularly suited to degrade polyethylene terephthalate, and material containing polyethylene terephthalate.
Claims22
An esterase variant which (i) has at least 75% identity to the full length amino acid sequence set forth in SEQ ID NO:
- 1, and (ii) has at least one amino acid substitution at a position selected from T157, T176, S65, P58, A62, A64, L67, A68, N85, T86, R89, D91, P93, R96, G128, M131, G133, G134, T153, P154, H156, A178, P179, H183, S206, P210 or N211, wherein the positions are numbered by reference to the amino acid sequence set forth in SEQ ID NO:
- 1, and (iii) exhibits increased polyester degrading activity compared to an esterase of SEQ ID NO:
- 1.
An esterase variant which (i) has at least 75% identity to the full length amino acid sequence set forth in SEQ ID NO:
- 1, and (ii) comprises at least one substitution or combination of substitutions selected from the group consisting of S65T, T157E/Q/N/G, T176H/N/Q, N211Q, F208W+V170I, Y92P+F208L, Y92P+F208W, T176H+F208W, V170I+A121S, V170I+A121S+S223A, F208W+T157Q, F208W+T157N, F208W+T157S, F208W+S65T and F208W+T157E, wherein the positions are numbered by reference to the amino acid sequence set forth in SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.