Mutant UDP-glycosyltransferase variants of Os79 or homologs thereof for T-2 toxin inactivation
AssigneeWisconsin Alumni Research Foundation
InventorsIvan Rayment, Karl Wetterhorn
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Abstract
Compositions and methods for increasing resistance to Fusarium infection in plants are provided herein. Polynucleotides, polypeptides, and expression constructs for expressing mutant UDP-glycosyltransferase proteins, plants comprising the polynucleotides, polypeptides or expression constructs, and methods of producing transgenic plants are also provided.
Claims31
A polynucleotide comprising a DNA sequence encoding a modified UDP-glycosyltransferase polypeptide, said polypeptide comprising at least two mutations relative to a wild-type UDP glycosyltransferase polypeptide, wherein the mutation renders the modified UDP glycosyltransferase polypeptide capable of glycosylating T-2 toxin, diacetoxyscirpenol (DAS), 4-acetyl-NIV (4-ANIV) or 4,15-diacetyl-NIV (4,15-di-ANIV) from Fusarium, wherein said modified UDP glycosyltransferase polypeptide comprises a mutation at positions corresponding to amino acids 122 and 123 of SEQ ID NO:2.
A method of increasing the resistance of a plant to Fusarium infection, comprising modifying a UDP-glycosyltransferase polypeptide of said plant, wherein said polypeptide comprises:
- a) at least a first mutation relative to a wild-type UDP glycosyltransferase polypeptide, and wherein said modified UDP glycosyl transferase polypeptide comprises a mutation at a position corresponding to amino acid 202 of SEQ ID NO:2;
- b) at least two mutations relative to a wild-type UDP glycosyltransferase polypeptide, and wherein said modified UDP glycosyl transferase polypeptide comprises a mutation at positions corresponding to amino acids 122 and 123 of SEQ ID NO:2;
- or
- c) at least three mutations relative to a wild-type UDP-glycosyltransferase polypeptide, and wherein said modified UDP glycosyltransferase polypeptide comprises a mutation at positions corresponding to amino acids 122, 123 and 202 of SEQ ID NO:2.
A polynucleotide comprising a DNA sequence encoding a modified UDP-glycosyltransferase polypeptide, said polypeptide comprising at least a first mutation relative to a wild-type UDP glycosyltransferase polypeptide, wherein the mutation renders the modified UDP glycosyltransferase polypeptide capable of glycosylating T-2 toxin, diacetoxyscirpenol (DAS), 4-acetyl-NIV (4-ANIV) or 4,15-diacetyl-NIV (4,15-di-ANIV) from Fusarium, wherein said modified UDP glycosyl transferase polypeptide comprises a mutation at a position corresponding to amino acid 202 of SEQ ID NO:2.
Dependencies are inferred from the claim text.