Non-covalent loading of plant picornavirus particles
AssigneeCase Western Reserve University
InventorsNicole Steinmetz, Daniel Popkin
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Abstract
A method of non-covalently loading a plant picornavirus is described. The method includes contacting a plant picornavirus in solution with a molar excess of a cargo molecule to load the plant picornavirus with the cargo molecule, and then purifying the loaded plant picornavirus. Examples of cargo molecules include imaging agents, antitumor agents, and antiviral agents. Loaded plant picornaviruses prepared in this manner can be used to delivering cargo molecule to cells.
Claims13
A method of loading a native plant picornavirus, comprising contacting a native plant picornavirus in solution with a molar excess of at least about 500 fold of a cargo molecule to load the plant picornavirus with the cargo molecule, and purifying the loaded plant picornavirus, wherein the cargo molecule is associated within the loaded plant picornavirus in a non-covalent manner, wherein the cargo molecule is loaded into the plant picornavirus via simple diffusion from the solution into the picornovirus.
Dependencies are inferred from the claim text.