CD133+ cells and method for expanding
AssigneeBoard of Regents, The University of Texas System
InventorsLarry Denner, Randall J. Urban, Yvonne Bodenburg
Life of this patent
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Abstract
Provided herein are isolated CD133+ cells, such as human CD133+ cells, that have been expanded in culture and populations of isolated CD133+ cells that have been expanded in culture. The cells express transcription factors, growth factors, or a combination thereof, at altered levels compared to a naturally occurring CD133+ cell. Also provided are methods, including methods for expanding CD133+ cells and methods for using CD133+ cells.
Claims24
An isolated human CD133+ cell population expressing at least one mRNA selected from FOXA2, HOXA1, MKI67, and PCNA at a higher level than naturally occurring human CD133+ cells, at least one mRNA selected from TCF7, KLF1, BMP3, GATA1, GATA3, IL18R1, and GDF10 at a lower level than naturally occurring human CD133+ cells, or a combination thereof.
A method for producing an expanded population of CD133+ cells, comprising:
- (a) providing a sample comprising isolated human CD133+ cells originating from a hematopoietic source;
- (b) incubating the CD133+ cells for at least 1 day in a first culture under conditions suitable for division of the CD133+ cells;
- (c) reisolating the CD133+ cells present in the first culture to result in reisolated CD133+ cells;
- (d) incubating the reisolated CD133+ cells for at least 1 day in a second culture under conditions suitable for division of the CD133+ cells, and reisolating the CD133+ cells present in the culture;
- and either
- (e) repeating the incubating and the reisolating of step (d) until the reisolated CD133+ cells are cultured at least 21 days after the reisolating of step (c) or
- (f) repeating the incubating and the reisolating of step (d) until at least 15% of the cells after the at least 1 day incubation in the second culture are CD133+.
A method comprising:
- providing human CD133+ cells that have been cultured ex vivo for at least 21 days, wherein the CD133+ cells originated from a hematopoietic source;
- culturing the human CD133+ cells for at least 1 day and no greater than 20 days under conditions suitable for division of the CD133+ cells;
- and
- isolating the CD133+ cells present in the culture wherein at least 15% of the cells after the culturing are CD133+.
Dependencies are inferred from the claim text.